Radar Online have said that Khloe is "much happier" as a single mum to their 10-month-old daughter True, according to multiple sources.
An insider told the publication that 34-year-old Khloe grew suspicious of Tristan, 27, over Christmas.
The reality star is said to have become convinced that the sportsman had cheated again when she spent the holiday in Calabasas with her family and he remained in Cleveland.
The pair's relationship was first rocked in April last year, when Tristan was accused of cheating on Khloe just days before the blonde beauty gave birth.
Khloe allowed her partner to be in the delivery room to welcome baby True in spite of the reports, and the couple ended up remaining together.
However, when True was four months old Khloe moved from Cleveland to Los Angeles with the tot, while Tristan remained in the Ohio city.
Despite this, fans believed that things were going well between the two, with Khloe taking to her Instagram page on Valentine's Day to share pictures of a huge red and pink bouquet of roses from the professional basketball player.
She captioned an image of the impressive floral display: "Daddy and baby True".
But the romantic day soon seemed to turn sour, with Khloe following up the post with multiple cryptic quotes that hinted that all is not well in her personal life.
One of the dark messages read: "Sometimes god breaks your heart to save your soul".
Another said: "She is a mess but she is a masterpiece."
Khloe has previously lashed out at speculation surrounding her and Tristan's long-distance relationship.
After she celebrated Thanksgiving away from her daughter's father, the star tweeted: "Y'all are reaching now.
"I've spent the past three years in Cleveland for Thanksgiving. Not sure why people are trying to create drama between my family and I."
Both Khloe and Tristan are yet to speak out on the most recent reports and The Sun Online have contacted Khloe's rep for comment.
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